Journal: British Journal of Cancer

Article Title: Prognostic significance of AMP-activated protein kinase expression and modifying effect of MAPK3/1 in colorectal cancer

doi: 10.1038/sj.bjc.6605846

Figure Lengend Snippet: P hosphorylated AMPK and p-MAPK3/1 expression in colorectal cancer. ( A ) Positive for p-AMPK cytoplasmic expression (arrowheads). ( B ) Negative for p-AMPK expression (white arrowheads). ( C ) Positive for p-MAPK3/1 nuclear expression (white arrows). ( D ) Negative for p-MAPK3/1 expression (block arrow). Stromal cells serve as an internal positive control for p-MAPK3/1 expression (arrow).

Article Snippet: Primary antibody against p-AMPK (rabbit monoclonal anti-phospho-AMPK α (Thr172) (40H9), 1 : 100 dilution; Cell Signaling Technology, Boston, MA, USA) was applied ( Ji et al , 2007 ; Contreras et al , 2008 ; Hadad et al , 2009 ; Vazquez-Martin et al , 2009 ; Zheng et al , 2009 ), and the slides were maintained at 4 °C for overnight, followed by rabbit secondary antibody (Vector Laboratories) (60 min), an avidin–biotin complex conjugate (Vector Laboratories) (60 min), diaminobenzidine (5 min) and methyl-green counterstain.

Techniques: Expressing, Blocking Assay, Positive Control

Journal: British Journal of Cancer

Article Title: Prognostic significance of AMP-activated protein kinase expression and modifying effect of MAPK3/1 in colorectal cancer

doi: 10.1038/sj.bjc.6605846

Figure Lengend Snippet: p-AMPK expression in colorectal cancer, and clinical, pathologic and molecular features

Article Snippet: Primary antibody against p-AMPK (rabbit monoclonal anti-phospho-AMPK α (Thr172) (40H9), 1 : 100 dilution; Cell Signaling Technology, Boston, MA, USA) was applied ( Ji et al , 2007 ; Contreras et al , 2008 ; Hadad et al , 2009 ; Vazquez-Martin et al , 2009 ; Zheng et al , 2009 ), and the slides were maintained at 4 °C for overnight, followed by rabbit secondary antibody (Vector Laboratories) (60 min), an avidin–biotin complex conjugate (Vector Laboratories) (60 min), diaminobenzidine (5 min) and methyl-green counterstain.

Techniques: Expressing, Significance Assay, Methylation, Mutagenesis

Journal: British Journal of Cancer

Article Title: Prognostic significance of AMP-activated protein kinase expression and modifying effect of MAPK3/1 in colorectal cancer

doi: 10.1038/sj.bjc.6605846

Figure Lengend Snippet: Kaplan–Meier curves for colorectal cancer-specific survival. ( A ) p-AMPK status and survival of colorectal cancer patients. The left panel includes all eligible cases, the middle panel includes p-MAPK3/1-positive cases, and the right panel includes p-MAPK3/1-negative cases. ( B ) p-MAPK3/1 status and survival of colorectal cancer patients. The left panel includes all eligible cases, the middle panel includes p-AMPK-positive cases, and the right panel includes p-AMPK-negative cases.

Article Snippet: Primary antibody against p-AMPK (rabbit monoclonal anti-phospho-AMPK α (Thr172) (40H9), 1 : 100 dilution; Cell Signaling Technology, Boston, MA, USA) was applied ( Ji et al , 2007 ; Contreras et al , 2008 ; Hadad et al , 2009 ; Vazquez-Martin et al , 2009 ; Zheng et al , 2009 ), and the slides were maintained at 4 °C for overnight, followed by rabbit secondary antibody (Vector Laboratories) (60 min), an avidin–biotin complex conjugate (Vector Laboratories) (60 min), diaminobenzidine (5 min) and methyl-green counterstain.

Techniques:

Journal: British Journal of Cancer

Article Title: Prognostic significance of AMP-activated protein kinase expression and modifying effect of MAPK3/1 in colorectal cancer

doi: 10.1038/sj.bjc.6605846

Figure Lengend Snippet: p-AMPK status in colorectal cancer and patient mortality

Article Snippet: Primary antibody against p-AMPK (rabbit monoclonal anti-phospho-AMPK α (Thr172) (40H9), 1 : 100 dilution; Cell Signaling Technology, Boston, MA, USA) was applied ( Ji et al , 2007 ; Contreras et al , 2008 ; Hadad et al , 2009 ; Vazquez-Martin et al , 2009 ; Zheng et al , 2009 ), and the slides were maintained at 4 °C for overnight, followed by rabbit secondary antibody (Vector Laboratories) (60 min), an avidin–biotin complex conjugate (Vector Laboratories) (60 min), diaminobenzidine (5 min) and methyl-green counterstain.

Techniques:

Journal: British Journal of Cancer

Article Title: Prognostic significance of AMP-activated protein kinase expression and modifying effect of MAPK3/1 in colorectal cancer

doi: 10.1038/sj.bjc.6605846

Figure Lengend Snippet: p-AMPK status and patient mortality in strata of p-MAPK3/1 status (upper rows) and p-MAPK3/1 status and patient mortality in strata of p-AMPK status (lower rows)

Article Snippet: Primary antibody against p-AMPK (rabbit monoclonal anti-phospho-AMPK α (Thr172) (40H9), 1 : 100 dilution; Cell Signaling Technology, Boston, MA, USA) was applied ( Ji et al , 2007 ; Contreras et al , 2008 ; Hadad et al , 2009 ; Vazquez-Martin et al , 2009 ; Zheng et al , 2009 ), and the slides were maintained at 4 °C for overnight, followed by rabbit secondary antibody (Vector Laboratories) (60 min), an avidin–biotin complex conjugate (Vector Laboratories) (60 min), diaminobenzidine (5 min) and methyl-green counterstain.

Techniques: